리도카인에 의한 p815 세포의 고사

Background and Objectives: Mast cells play a major role in pathogenesis of allergic rhinitis. Lidocaine is widely used as a short-acting local anesthetic and an anti-arrhythmic agent. Lidocaine also has anti-inflammatory properties by inhibiting the effect of inflammatory cytokines. Although lidocaine is reported to suppress allergic reactions, there have been no report about lidocaine’s direct inhibitory effect on mast cells. The purposes of this study are to identify whether lidocaine directly induces apoptosis of mast cells in vitro and know the pathway of apoptosis. Materials and Methods: After culture of p815 cells, mouse mastocytoma cells, the cells were treated with 2.5 μM, 5 μM lidocaine for 24, 48 and 72 hours and then LD50 of p815 cells was calculated by MTT β 3- [4, 5-di- methylthiazol-2-yl] -2,5-diphenyl-tetrazolium bromide) assay. For identification of apoptosis of p815 cells, flow cytometric analysis in monclonal antibody to annexin V and cell cycle was done. We also estimated the expression of procaspase-3, procaspase-8, procaspase-9, AIF (apoptosis inducing factors) to know the pathway of apoptosis. Results: The LD₅₀ of p815 cells is 3.70 μM after lidocaine treatment for 24 hours, 1.82 μM for 48 hours and 1.72 μM for 72 hours. Lidocaine induced apoptosis in time and dose dependent manner. Procaspase-3 expression was significantly decreased while procaspase-8, procaspase-9 and AIF were not changed. Conclusion: Our results suggest lidocaine can induce apoptosis in mast cells via caspase-3 dependent pathway. Further studies on the pathway of apoptosis and clinical use will be needed. (J Clinical Otolaryngol 2005;16:247–252)