Journal of Clinical Otolaryngology Head and Neck Surgery
The Busan, Ulsan, Gyeoungnam Branch of Korean Society of Otolaryngology-Head and Neck Surgery
원저

리도카인에 의한 p815 세포의 고사

이일우1, 이현순1, 조규섭1, 박희영2, 곽종영3, 노환중1,*
Il-Woo Lee1, Hyun-Sun Lee1, Kyu-Sup Cho1, Hee-Young Park2, Jong-Young Guak3, Hwan-Jung Roh1,*
1부산대학교 의과대학 이비인후과학교실
2의학연구소
3동아대학교 의과대학 생화학교실
1Department of Otolaryngology, College of Medicine, Pusan National University, Busan, Korea
2Medical Research Institute, College of Medicine, Pusan National University, Busan, Korea
3Department of Biochemistry, College of Medicine, Dong-A University, Busan, Korea
*교신저자: 노환중, 602-739 부산광역시 서구 아미동 1가 10 부산대학교 의과대학 이비인후과학교실 전화: (051) 240-7333·전송: (051) 248-1248 E-mail: rohhj@pusan.ac.kr

© Copyright 2005 The Busan, Ulsan, Gyeoungnam Branch of Korean Society of Otolaryngology-Head and Neck Surgery. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Aug 15, 2005; Accepted: Sep 16, 2005

Published Online: May 31, 2020

ABSTRACT

Background and Objectives: Mast cells play a major role in pathogenesis of allergic rhinitis. Lidocaine is widely used as a short-acting local anesthetic and an anti-arrhythmic agent. Lidocaine also has anti-inflammatory properties by inhibiting the effect of inflammatory cytokines. Although lidocaine is reported to suppress allergic reactions, there have been no report about lidocaine’s direct inhibitory effect on mast cells. The purposes of this study are to identify whether lidocaine directly induces apoptosis of mast cells in vitro and know the pathway of apoptosis. Materials and Methods: After culture of p815 cells, mouse mastocytoma cells, the cells were treated with 2.5 μM, 5 μM lidocaine for 24, 48 and 72 hours and then LD50 of p815 cells was calculated by MTT β 3- [4, 5-di- methylthiazol-2-yl] -2,5-diphenyl-tetrazolium bromide) assay. For identification of apoptosis of p815 cells, flow cytometric analysis in monclonal antibody to annexin V and cell cycle was done. We also estimated the expression of procaspase-3, procaspase-8, procaspase-9, AIF (apoptosis inducing factors) to know the pathway of apoptosis. Results: The LD50 of p815 cells is 3.70 μM after lidocaine treatment for 24 hours, 1.82 μM for 48 hours and 1.72 μM for 72 hours. Lidocaine induced apoptosis in time and dose dependent manner. Procaspase-3 expression was significantly decreased while procaspase-8, procaspase-9 and AIF were not changed. Conclusion: Our results suggest lidocaine can induce apoptosis in mast cells via caspase-3 dependent pathway. Further studies on the pathway of apoptosis and clinical use will be needed. (J Clinical Otolaryngol 2005;16:247–252)

Keywords: 비만세포; 세포고사; 리도카인; 알레르기비염
Keywords: Wast cell; Apoptosis; Lidocaine; Allergic rhinitis