알레르기비염 동물 모델에서 Budesonide, N-Acetyl-Aspartyl- Glutamate 및 Cyclosporin A 국소투여 후 비강내 아미노산 농도의 변화：미세투석법을 이용한 측정

Background and Objectives: This study aimed to measure the concentration (conc) of 19 amino acids (AAs) in the nasal cavity, to evaluate the difference of AAs conc among normal, allergic nasal mucosa, and allergic mucosa after intranasal application of budesonise, N-acetyl-aspartyl-glutamate (NAAGA), and cyclosporin (CsA) in guinea pigs. Materials and Method: Experimentally induced nasal allergy model was developed with ovalbumin in Dunkin-Hartely guinea pigs. Microdialysis was done in the inferior turbinate submucosa of normal control (C) , allergic group (A) , allergic group with intranasal application of budesonide (AS) , allergic group with intranasal application of NAAGA (AN) and allergic group with intranasal application of CsA (AC) . Results: All 19 AAs were validated at various conc in the nasal cavity. Glutamate and GABA conc were significantly higher in A than in C group. The conc serine, arginine, tyrosine, isoleucine, leucine, phenylalanine in AS group were significantly lower than those in A and C groups, whereas GABA conc was significantly higher in AS group than in A and C groups. The conc of glutamate, glycine, alanine, methionine, leucine, lysine, phenylalanine in A group were significantly higher than those in AN group, whereas tyrosine conc was reversed. There was no significant changes of AAs conc between A and AC groups. Conclusion: These results suggest that glutamate can be a novel amino acid neurotransmitter of parasympathetic nerve and GABA can act as an inhibitor of cholinergic parasympathetic nerve in the nasal cavity. (J Clinical Otolaryngol 2005;16:96–104)