Journal of Clinical Otolaryngology Head and Neck Surgery
The Busan, Ulsan, Gyeoungnam Branch of Korean Society of Otolaryngology-Head and Neck Surgery
원저

알레르기비염 동물 모델에서 Budesonide, N-Acetyl-Aspartyl- Glutamate 및 Cyclosporin A 국소투여 후 비강내 아미노산 농도의 변화:미세투석법을 이용한 측정

노환중1,*, 장민혁1, 이현순1, 조규섭1
Hwan-Jung Roh1,*, Min-Hyeog Jang1, Hyun-Sun Lee1, Kyu-Sup Cho1
1부산대학교 의과대학 이비인후과학교실
1Department of Otolaryngology, College of Medicine, Busan National University, Busan, Korea
*교신저자: 노환중, 602-739 부산광역시 서구 아미동 1가 10 부산대학교 의과대학 이비인후과학교실 전화: (051) 240-7333·전송: (051) 246-8668 E-mail: rohhj@pusan.ac.kr

© Copyright 2005 The Busan, Ulsan, Gyeoungnam Branch of Korean Society of Otolaryngology-Head and Neck Surgery. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Apr 20, 2005; Accepted: May 30, 2005

Published Online: May 31, 2020

ABSTRACT

Background and Objectives: This study aimed to measure the concentration (conc) of 19 amino acids (AAs) in the nasal cavity, to evaluate the difference of AAs conc among normal, allergic nasal mucosa, and allergic mucosa after intranasal application of budesonise, N-acetyl-aspartyl-glutamate (NAAGA), and cyclosporin (CsA) in guinea pigs. Materials and Method: Experimentally induced nasal allergy model was developed with ovalbumin in Dunkin-Hartely guinea pigs. Microdialysis was done in the inferior turbinate submucosa of normal control (C) , allergic group (A) , allergic group with intranasal application of budesonide (AS) , allergic group with intranasal application of NAAGA (AN) and allergic group with intranasal application of CsA (AC) . Results: All 19 AAs were validated at various conc in the nasal cavity. Glutamate and GABA conc were significantly higher in A than in C group. The conc serine, arginine, tyrosine, isoleucine, leucine, phenylalanine in AS group were significantly lower than those in A and C groups, whereas GABA conc was significantly higher in AS group than in A and C groups. The conc of glutamate, glycine, alanine, methionine, leucine, lysine, phenylalanine in A group were significantly higher than those in AN group, whereas tyrosine conc was reversed. There was no significant changes of AAs conc between A and AC groups. Conclusion: These results suggest that glutamate can be a novel amino acid neurotransmitter of parasympathetic nerve and GABA can act as an inhibitor of cholinergic parasympathetic nerve in the nasal cavity. (J Clinical Otolaryngol 2005;16:96–104)

Keywords: 알레르기비염; Budesonide; N-acetyl-aspartyl-glutamate; Cyclosporin; 아미노산
Keywords: Rhinitis; Allergic; Budesonide; N-acetyl-aspartyl-glutamate; Cyclosporin; Amino acid