중이 진주종조직에서 ICAM-1, VCAM-1, ELAM-1의 발현에 관한 연구

Background and Objectives: Cholesteatoma is associated with an intense inflammatory reaction with resultant destruction of tissue and bone. To evaluate the potential role of several adhesion molecules in the pathogenesis of cholesteatoma and its bone destruction, the authors studied mRNA expression of three adhesion molecules in the cholesteatoma tissue. Materials and Methods: We investigated mRNA expression of adhesion molecules such as intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and endothelial derived leukocyte adhesion molecule-1 (ELAM-1) in 20 cholesteatoma tissues as well as in the normal postaural skins using reverse transcription-polymerase chain reaction (RT-PCR). To evaluate the relationship between degree of bone destruction and adhesion molecules, we collected different portions of cholesteatoma according to the destructive status of middle ear bony structure in 5 cases and examined the expression of the mentioned factors to be compared. Results: 0f 20 cholesteatoma, eighteen (90%) showed ELAM-1 and VCAM- 1 gene expression and 8 (40%) showed ELAM-1 mRNA. There were much lower gene expressions in the normal postaural skin than the cholesteatoma. Conclusion: These results suggest that ICAM-1 and VCAM-1 play a central role in the regulation of the inflammatory disorders observed in cholesteatoma including migration, adhesion and proliferation of inflammatory cells. (J Clinical Otolaryngol 2002;13:65–72)