단기간 Aldehyde에 Tanning 처리한 교원질 해면의 조직 반응

Treating of the collagen material by chemical preservatives is a procedure to make a homograft material in otologic surgery. Chemical treatment eliminates the antigenicity of allo-or xenograft materials and enhances their physical strength for molding. To evaluate the toxicity of chemical treatment through histological method, bovine collagen sponges treated with 0.5% buffered glutaraldehyde or 4% formaldehyde solution. These sponges were inserted into subcutaneous pockets of the rats and removed after 2 and 5 days. They were stained with hematoxilin-eosin and analyzed by light microsocopy. Quantitative morphometry was done. Two days after implantation, the morphology showed cellular reaction around the all rinsed collagen sponge. Inflammatory cells were presented in the surface of the sponge. At five days after implantation, more pronounced cellular reactions were noticed around the rinsed sponge. Collagen sponge without rinsing showed markedely melted architecture with inflammatory cell infiltration. (J Clinical Otolaryngol 1999;10:259–263)