진주종성 중이염 환자의 진주종에서 iNOS 존재

Background and Objectives: Bone resorption and hyperkeratosis are the most characteristic findings of chronic otitis media with cholesteatoma. Overexpression of IL-1 in cholesteatoma was reported having a significant role in the pathogenesis of cholesteatoma. Also, overproduction of nitric oxide (NO) stimulated the production of the cyclooxygenase and PGE2. Therefore, NO and inducible nitric oxide synthase (NOS) may be suggested having a role of the pathogenesis of cholesteatoma. This study was designed to identify the existence of iNOS in human cholesteatoma. Materials and Methods: Ten fresh human cholesteatoma tissues and one postauricular skin were used. Dot and Western blot analysis were used for detection of iNOS. Results: Dot blot showed different degrees of response. The bands of iNOS about 137 kDa area existed in the Western blot analysis. Conclusion: These findings suggest that iNOS may present in human cholesteatoma and NO may have play a role in pathogenesis of chronic otitis media with cholesteatoma. (J Clinical Otolaryngol 1999;10:190–194)