포르말린 고정 후 파라핀에 포매된 조직에서
Published Online: May 31, 2020
ABSTRACT
One of the most dramatic changes in the study of cancer is the usage of PCR technique for the research of genetic alterations. However, as the PCR technique amplifies DNA from small cell populations, there’s always a problem of tissue heterogeneity. If PCR is done in specimens composed of multiple cell types such as normal epithelial cells, inflammatory cells, fat cells and cancer cells, the recovered DNA will reflect an average from all cell types and not the specific DNA of cancer cells. To overcome this problem, tissue microdissection technique was used in formalin-fixed, paraffin-embedded specimens. PCR was done with routine 10-ju slides of -specimens after mierodissection, which resulted in success. This technique is useful in DNA studies of small lesions and tissue samples of multiple cell types.