원저

포르말린 고정 후 파라핀에 포매된 조직에서

박재율1, 유정운1, 신승헌1, 손진호1
Jae Yul Park1, Jung Woon You1, Seung Heun Shin1, Jin Ho Sohn1
Author Information & Copyright
1대구 효성 가톨릭대학교 의과대학 이비인후과 학교실
1Department of Otolaryngology, School of Medicine, Catholic University of Taegu-Hyosung, Taegu, Korea

© Copyright 1997 The Busan, Ulsan, Gyeoungnam Branch of Korean Society of Otolaryngology-Head and Neck Surgery. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Published Online: May 31, 2020

ABSTRACT

One of the most dramatic changes in the study of cancer is the usage of PCR technique for the research of genetic alterations. However, as the PCR technique amplifies DNA from small cell populations, there’s always a problem of tissue heterogeneity. If PCR is done in specimens composed of multiple cell types such as normal epithelial cells, inflammatory cells, fat cells and cancer cells, the recovered DNA will reflect an average from all cell types and not the specific DNA of cancer cells. To overcome this problem, tissue microdissection technique was used in formalin-fixed, paraffin-embedded specimens. PCR was done with routine 10-ju slides of -specimens after mierodissection, which resulted in success. This technique is useful in DNA studies of small lesions and tissue samples of multiple cell types.

Keywords: PCR; Tissue microdissection; Paraffin-embedded specimen