원저

타액선 질환 치료에서의 A형 보툴리눔 독소 주입

박노선1, 장동식1, 조진생1, 박승구1, 김지찬1, 이호윤1,*
No-Seon Park1, Dong-Sik Chang1, Chin-Saeng Cho1, Seung-Gu Park1, Ji Chan Kim1, Ho Yun Lee1,*
Author Information & Copyright
1을지대학교 의과대학 이비인후과학교실
1Department of Otorhinolaryngology-Head & Neck Surgery, Colleage of Medicine, Eulji University, Daejeon, Korea
*교신저자: 이호윤, 302-799 대전광역시 서구 둔산서로 95 을지대 학교 의과대학 이비인후과학교실 전화: (042) 611-3133·전송: (042) 611-3136 E-mail: hoyun1004@gmail.com

© Copyright 2014 The Busan, Ulsan, Gyeoungnam Branch of Korean Society of Otolaryngology-Head and Neck Surgery. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Sep 18, 2014; Revised: Oct 02, 2014; Accepted: Nov 07, 2014

Published Online: May 31, 2020

ABSTRACT

Background and Objectives: Temporalis fascia and cartilage are well-known materials for the restoration of defective tympanic membrane. Common organisms of postoperative infection in tympanoplasty are S. aureus and P. aeruginosa. This study was done to understand the resistance of cartilage and fascia to extracellular bacterial proteolytic enzyme of the pathogens. Materials and Methods:S. aureus, P. aeruginosa, E. col were put in LB broth and Nutrient broth, incubated each other during 24 hours under 37 °C. We concentrated culture supernatants on ammonium sulphate precipitation, and dialyzing it with normal saline. Extracellular enzyme with same sized cartilage and temporalis fascia tissue were nourished during 48 hours. The amounts of degradated proteins were measured, then proteins on molecular weight enforced by SDS-PAGE were classified. The activities of proteolytic enzyme by enforcement of zymography were detected. Results: We showed the proteolytic activities of cartilage and temporalis fascia by the reaction of extracellular enzyme. S. aureus had the most potent destructive power and the cartilage was more resistant to bacterial extracellular proteolysis in S. aureus than fascia. Conclusions: It could suggest that the cartilage is good for the graft material in aspect of the resistance to extracellular bacterial proteolysis. (J Clinical Otolaryngol 2014;25:150–155)

Keywords: 고실성형술; 이주 연골; 측두 근막; 단백 분해; 만성 중이염
Keywords: Cartilage; Fascia; Tympanoplasty; Proteolysis; Chronic otitis media