위산 역류 질환 동물모델의 확립에 대한 예비결과보고

Backgrounds: To investigate the pathophysiolosy of laryngopharyngeal reflux disease (LPRD), an experimental animal model is required. Therefore, we tried to make animal model of laryngopharyngeal reflux, modifying Omura’s method. Methods: In the first phase of study, we used Omura’s method to make animal model, ligating forestomach through purse-string suture. In the modified group A, pyrolic stenosis was made with 20F nelaton catether after 2 mm cut of it and forestomach was ligated by purse-string suture. In the modified group B, pyrolic stenosis was made with 20F nelaton catether and forestomach was ligated by purse-string suture. Results: The survival rate of Omura’s animal model was 0% (0/36), modified group A was 24% (5/21), and modified group B was 44% (8/18). Marked ulceration and mild erosion in the distal esophagus were observed in histological examination of modified group A, B, respectively. However, there was no definite inflammatory findings and squamous metaplasia in the larynx. Conclusions: The modified A and B methods were useful animal model of gastroesophageal reflux compared to original Omura’s method. However, further investigation was required to make an animal model of laryngopharyngeal reflux by these methods. (J Clinical Otolaryngol 2013;24:73–79)