원저

Can the Palatine Tonsil be a Source of Mesenchymal Stem Cells with Immunomodulatory Property?

Yoon Se Lee1, Hee-Young Park2, Yun Sung Lim2, Jin-Choon Lee2, Soo-Geun Wang2, Jin Sup Jung3, Byung-Joo Lee2,*
Author Information & Copyright
1Department of Otorhinolaryngology-Head and Neck Surgery, Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan, Korea
2Department of Otorhinolaryngology-Head and Neck Surgery, Pusan National University Hospital and Medical Research Institute, Busan, Korea
3Department of Physiology, School of Medicine, Pusan National University, Busan, Korea
*교신저자: 이병주,602-739 부산광역시 서구 구덕로 Г79 부산대학교 의과대학 이비인후과학교실 전화: (051) 240-7335 · 전송: (051) 246-8668 · E-mail: voicelee@pusan.ac.kr

© Copyright 2012 The Busan, Ulsan, Gyeoungnam Branch of Korean Society of Otolaryngology-Head and Neck Surgery. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Mar 08, 2012; Revised: Mar 27, 2012; Accepted: Apr 30, 2012

Published Online: May 31, 2020

ABSTRACT

Background and Objectives: Mesenchymal stem cells (MSCs) are multi potent cells that can differentiate into various cell types and are isolated from various other human adult and fetal tissues. Our study is to isolate palatine tonsil-derived MSCs (T-MSCs) and evaluate their differentiation potential and immunomodulatory effects, compared with bone marrow-derived MSCs (BM-MSCs). Methods: T-MSCs were isolated from human palatine tonsil. The expression of surface markers of T-MSCs was assessed by flow cytometric analysis. Differentiation potential of T-MSCs was analyzed histochemically and by the expression of lineage-related marker genes. Immunomodulatory effects were evaluated by mixed lymphocyte reactions (MLR) and mitogen proliferation assays with phytohemagglutinin (PHA). Results: T-MSCs were isolated from palatine tonsil and displayed a similar morphology to BM-MSCs. T-MSCs were negative for CD 31?CD45, CD117, HLA-DR and positive for CD44, CD73, CD90, and CD105 in flow cytometric analysis. The expression of surface phenotypes and differentiation potential of T-MSCs exhibited the similar finding as BM-MSCs. T-MSCs showed the significant inhibition of the proliferation of T cells stimulated by allergenic T cells in MLR study and by PHA stimuli in mitogen proliferation assay and also significant decrease of the secretion of TNF-a and IFN-γ (p <0.05). The HLA-G5 secretion by T-MSCs showed the significant increase in MLR and mitogen proliferation assays (p<0.05). T-MSCs do satisfy the phenotypical and functional definition of MSCs. T-MSCs also displayed immuno- modulatory effects that were associated with inhibiting T-cell proliferation, decreasing soluble factors, and increasing HLA-G5 secretion in response to various stimuli. Conclusions: We propose that T-MSCs provide a good alternative for allogeneic MSCs in therapeutic applications. (J Clinical Otolaryngol 2012;23:90–100)

Keywords: Palatine tonsil; Mesenchymal stem cell; Immune modulation


(최종) 35권 4호 Quiz 및 정답, 해설


*공지* 

출제 지문 오류로 인해 35권 4호 Quiz 문제를 다시 알립니다.

정답은 최종 1번으로 기존 출제된 지문에는 정답이 없으나,

참여와 관심에 감사의 마음을 담아 응모하신 모든 분들께 상품은 

지급해 드리도록 하겠습니다.  감사합니다.

 

다음은 갑상선 로봇수술 사진이다. 다음 설명 중 틀린 것은?

 

1. 이 접근법은 Mental nerve injury의 가능성이 있어 mental nerve를 찾아 위치를 확인 후 수술한다.

2. 일반적으로 이산화탄소를 주입해서 공간을 확보하므로 합병증으로 CO2 embolism 발생 가능성이 있다.

3. 다른 부위로의 접근법에 비해 절제하고자 하는 부위에 빨리 도달할 수 있다.

4. 합병증으로 다른 부위로의 접근법에 비해 수술 부위 감염 가능성이 있다.

5. 합병증으로 다른 부위 접근법에 비해 구강이나 턱, 경부 등에 피부 천공이나 화상 등이 드물게 발생할 수 있다.

 

정답 ①

해설

Transoral thryoidectomy의 합병증으로 다른 접근법보다 CO2 embolism,
surgical site infection, skin perforation,burn risk rate 가 높다.
Metal nerve injury risk가 있어 구강전절절개를 safety zone에 해야 한다.

Ref

Kyung Tae, Clin Exp Otorhinolaryngol. 2021; 14(2): 169-178.
Complications of Transoral Thyroidectomy: Overview and Update


I don't want to open this window for a day.