Interleukin-1β가 중이 진주종의 골파괴에 미치는 영향

Background and Objectives: Cholesteatoma is characterized by temporal bone erosions resulting in fatal intracranial complications. Osteoclasts are the principal cell of bone resorption playing a major role in focal bone erosion associated with cholesteatoma. Inflammatory cytokines such as interleukins and tumor necrosis factor are implicated in osteoclastic bone resorption. This study was conducted in order to investigate the effect of interleukin-1β(IL-1β) on bone resorption in cholesteatoma. Materials and Methods: The concentration of IL-1β in cholesteatoma epithelium was measured by enzyme linked immunosorbent assay. The effects of osteoclastogenesis and osteoclastic bone resoption were measured in receptor activator for NF-κB ligand-induced mouse osteoclast cultures and by osteoclastic bone resorption assay. Results: The concentration of IL-1β in cholesteatoma epithelium was significantly elevated compared with control. Treatment with IL-1β (10 ng/mL and 100 ng/mL) significantly increased osteoclastogenesis. The resorption surface area on dentin slices were significantly increased by osteoclasts which were stimulated with IL-1β(10 ng/mL). IL-1 receptor antagonist (10 ug/mL) blocked the upregulatory effect of IL-1β on osteoclastogenesis. Conclusion: These results suggest that the increased IL-1β produced by cholesteatoma epithelium directly stimulates osteoclasts leading to focal bone erosion in cholesteatoma. (J Clinical Otolaryngol 2008;19:177-182)