Prodigiosin이 두경부 종양세포주의 TRAIL매개 세포자멸사에 미치는 효과

Background and Objectives: Prodigiosin (PG) is a red pigment produced by Seixatia marcescens with immu-nosuppressive and apoptotic activities. The purpose of this study was to evaluate the apoptotic effect and mechanism of PG on head and neck cancer, and show possibility of combination treatment with tumor necrosis factor related apoptosis inducing ligand (TRAIL) in TRAIL resistant cancer cells. Materials and Methods: Three head and neck cancer cell lines (AMC-HN4, AMC-HN6, AMC-HN9) were treated with PG and TRAIL. In order to know the mechanism of apoptosis, caspase 3 activity and apoptosis related protein expression were monitored by Western blotting. Results: TRAIL and PG induced apoptosis of AMC-HN9 cancer cells in dose- dependent manner. In AMC-HN4 cancer cells cotreated with PG and TRAIL, apoptosis was increased with up- regulation of PARP and DR5 expression and down-regulation of inactive form of caspase 3. Conclusions: PG effectively recovers TRAIL sensitivity in AMC-HN4, TRAIL and PG-resistant cancer cells, via DR5 up-regulation. PG alone or combination therapy with TRAIL could be a potent new strategy for treating head and neck cancer. (J Clinical Otolaryngol 2008;19:63–71)