원저

비용에서 세포고사체 및 Bax와 Bcl-2의 발현에 관한 연구

강일규1, 황유진2, 하종수1, 김동규1, 김선태1,*
Il-Gyu Kang1, Yu-Jin Hwang2, Jong-Su Ha1, Dong-Gyu Kim1, Seon-Tae Kim1,*
Author Information & Copyright
1가천의과대학교 길병원 이비인후-두경부외과학교실
2분자생물학교실
1Department of Otolaryngology-Head and Neck Surgery, Gil Medical Center, Gachon Medical School, Incheon, Korea
2Molecular Biololy Laboratory, Gil Medical Center, Gachon Medical School, Incheon, Korea
*교신저자: 김선태, 405-760 인천광역시 남동구 구월동 1198 가천의과대학교 길병원 이비인후-두경부외과학교실 전화: (032) 460-3764·전송: (032) 467-9044 E-mail: rhinokim2002@hanmail.net

© Copyright 2005 The Busan, Ulsan, Gyeoungnam Branch of Korean Society of Otolaryngology-Head and Neck Surgery. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Sep 08, 2005; Accepted: Oct 14, 2005

Published Online: May 31, 2020

ABSTRACT

Background and Objectives: Apoptosis is a programmed cell death and an active regulatory response by physiologic stimuli. Eosinophils are predominanat cells in the nasal polyp. However whether the apoptosis of the eosinophils could contribute to the increased numbers of eosinophils in the nasal polyp is not completely known. We have studied to reveal the relationship between the increase in the number of eosinophils and apoptosis. Materials and Methods: Subjects are 15 non-allergic nasal polyps and 10 normal inferior turbinates. We have used TUNEL method for apoptosis study and RT-PCR was used for the Bax and Bcl-2. Results: The apoptotic cell ratio (apoptotic cell numbers/total cell numbers) was 0.147 in normal turbinate compared to 0.05 in nasal polyp (p=0.056). The apoptotic cell ratio was higher in normal turbinate compared to that of nasal polyp. The apoptotic bodies of eosinophils in nasal polyp were very scanty. The RT-PCR showed that Bax was increased in normal turbinate, however Bcl-2 was increased in nasal polyp. These results suggest that the apoptosis of eosinophils in nasal polyp was decreased compared to the normal turbinate. Conclusion: Apoptosis may be one of the contributing factors for the increased numbers of eosinophils in nasal polyp. (J Clinical Otolaryngol 2005;16:264–269)

Keywords: 비용; 세포고사; Bax; Bcl-2
Keywords: Nasal polyps; Apoptosis; Bax; Bcl-2