비용에서 세포고사체 및 Bax와 Bcl-2의 발현에 관한 연구

Background and Objectives: Apoptosis is a programmed cell death and an active regulatory response by physiologic stimuli. Eosinophils are predominanat cells in the nasal polyp. However whether the apoptosis of the eosinophils could contribute to the increased numbers of eosinophils in the nasal polyp is not completely known. We have studied to reveal the relationship between the increase in the number of eosinophils and apoptosis. Materials and Methods: Subjects are 15 non-allergic nasal polyps and 10 normal inferior turbinates. We have used TUNEL method for apoptosis study and RT-PCR was used for the Bax and Bcl-2. Results: The apoptotic cell ratio (apoptotic cell numbers/total cell numbers) was 0.147 in normal turbinate compared to 0.05 in nasal polyp (p=0.056). The apoptotic cell ratio was higher in normal turbinate compared to that of nasal polyp. The apoptotic bodies of eosinophils in nasal polyp were very scanty. The RT-PCR showed that Bax was increased in normal turbinate, however Bcl-2 was increased in nasal polyp. These results suggest that the apoptosis of eosinophils in nasal polyp was decreased compared to the normal turbinate. Conclusion: Apoptosis may be one of the contributing factors for the increased numbers of eosinophils in nasal polyp. (J Clinical Otolaryngol 2005;16:264–269)