Subventricular Zone과 Olfactory Bulb에서 신경줄기세포의 배양에 대한 연구

Background: Neural stem cells are present in adult brain as well as fetal brain and have capacity for self-renewal and pluripotenciality in vitro culture. Therefore, they can be used to treat neurological damages in various degenerative disorders. Objectives: This study was designated to isolate neural stem cells from the central nervous system of neonatal mice, and to examine the effect of brain-derived neurotrophic factor (BDNF) on glial and neuronal differentiation in mouse neural stem cells. Materials and Methods: Cells isolated from the subventricular zone (SVZ) and the olfactory bulb of the 2—3 day old mice brain were grown as neurospheres in the presence of leukemia inhibitoy factor and fibroblast growth factor-2. RT-PCR analysis showed that Oct-4 was expressed in neural stem cells, of which expression was markedly decreased by induction of differentiation, and that neural differentiation of neural stem cells induced expression of various neural markers. Results: Neural stem cells were differentiated into neuronal and glial cells. BDNF increased neurite outgrowth during differentiation of neural stem cells. Western blot analysis demonstrated that BDNF induced expression of synaptophysin, a neuronal marker, and inhibited expression of glial fibrillry acidic protein, a glial marker. Conclusions: These results indicated that neural stem cells can be isolated from the subventricular zone and the olfactory bulb of neonatal mice, and that BDNF induces neural differentiation and inhibits glial differentiation in neural stem cells. (J Clinical Otolaryngol 2004;15:227–233)