원저

Subventricular Zone과 Olfactory Bulb에서 신경줄기세포의 배양에 대한 연구

이일우1, 이현순1, 정진섭2, 박희영3, 노환중1,*
IL-Woo Lee1, Hyun-Sun Lee1, Jin-Sup Jung2, Hee-Young Park3, Hwan-Jung Roh1,*
Author Information & Copyright
1부산대학교 의과대학 이비인후과학교실
2생화학교실
3부산대학교병원 의학연구소
1Department of Otolaryngology, Pusan National University Hospital, Busan, Korea
2Physiology, College of Medicine, Pusan National University, Busan, Korea
3Medical Research Institute, Pusan National University Hospital, Busan, Korea
*교신저자: 노환중, 602-739 부산광역시 서구 아미동 1가 10 부산대학교 의과대학 이비인후과학교실 전화: (051) 240-7333·전송: (051) 246-8668 E-mail: rohhj@pusan.ac.kr

© Copyright 2004 The Busan, Ulsan, Gyeoungnam Branch of Korean Society of Otolaryngology-Head and Neck Surgery. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Oct 01, 2004; Accepted: Nov 30, 2004

Published Online: May 31, 2020

ABSTRACT

Background: Neural stem cells are present in adult brain as well as fetal brain and have capacity for self-renewal and pluripotenciality in vitro culture. Therefore, they can be used to treat neurological damages in various degenerative disorders. Objectives: This study was designated to isolate neural stem cells from the central nervous system of neonatal mice, and to examine the effect of brain-derived neurotrophic factor (BDNF) on glial and neuronal differentiation in mouse neural stem cells. Materials and Methods: Cells isolated from the subventricular zone (SVZ) and the olfactory bulb of the 2—3 day old mice brain were grown as neurospheres in the presence of leukemia inhibitoy factor and fibroblast growth factor-2. RT-PCR analysis showed that Oct-4 was expressed in neural stem cells, of which expression was markedly decreased by induction of differentiation, and that neural differentiation of neural stem cells induced expression of various neural markers. Results: Neural stem cells were differentiated into neuronal and glial cells. BDNF increased neurite outgrowth during differentiation of neural stem cells. Western blot analysis demonstrated that BDNF induced expression of synaptophysin, a neuronal marker, and inhibited expression of glial fibrillry acidic protein, a glial marker. Conclusions: These results indicated that neural stem cells can be isolated from the subventricular zone and the olfactory bulb of neonatal mice, and that BDNF induces neural differentiation and inhibits glial differentiation in neural stem cells. (J Clinical Otolaryngol 2004;15:227–233)

Keywords: 줄기 세포; 후구; 배양
Keywords: Stem cell; Olfactory bulb; Culture