비인강암에서 중합효소반응을 이용한 유두종 바이러스의 검출

Background and Objectives: Nasopharyngeal carcinoma (NPC) is an unique epithelial malignancy which occurs at a high frequency in certain regions of Southeast Asia. Epstein-Barr virus (EBV) has been proposed to be closely associated with NPC. Human papillomavirus (HPV) is associated with a proportion of upper aero- digestive tract carcinomas. We hypothesized that HPV might also contribute to the pathogenesis of NPC. This study attempted to detect HPV DNA in nasopharyngeal carcinoma by polymerase chain reaction (PCR) . Materials and Methods: We reviewed thirty patients with nasopharyngeal carcinoma from 1995 to 2000 at the Department of Otolaryngology, Pusan National University Hospital. Thirty paraffin-embedded tissues were selected to PCR using type-specific primer pairs. Control group was selected twenty normal adenoid tissue. Results: HPV DNA was detected in 3/30 (10%) of NPC specimens, and in none of control specimens. WHO class I nasopharyngeal carcinomas (three cases) were all HPV type 6 positive. Conclusion: These results suggest that HPV type 6 may also be involved in the carcinogenesis of EBV-negative squamous cell nasopharyngeal carcinoma. (J Clinical Otolaryngol 2004;15:103–108)