진주종 유래 섬유아세포에서의 Insulin-Like Growth Factor I과 Binding Protein의 역할

Background and Objectives: Insulin like growth factor-I is an important growth regulator for epidermis and its activity is regulated by specific insulin like growth factor binding protein. Authors observed the expression of mRNA of Insulin-like Growth FactorI (IGF-I) and mRNA of Insulin-like Growth Factor Binding Protein (IGFBP) in fibroblast of patient with cholesteatoma to find out the function of fibroblast in the progress of cholesteatoma. Materials and Method: During the operation in middle ears, the cholesteatoma and normal skin tissue were extracted from 10 patients with Cholesteatoma. Some cytokines were added such as Interferon β, Interferon γ and TNF α to those extraced fibroblast. We analyzed the expression of IGF-I, IGFBP-3, and IGFBP-5 by semi-quantative RT-PCR. Results: After stimulation by inflammatory cytokine, the expression of IGF-I from cholesteatoma was significantly increased ,while the expression of IGFBP-5 was significantly reduced. Conclusion: These results indicate that cholesteatoma fibroblast could play an important role in the proliferation of cholesteatoma tissue. (J Clinical Otolaryngol 2003;14:113-117)