배양 비점막 상피세포에서 IFN-γ와 IL-4에 의한 ICAM-1의 발현양상

Background and Objectives: Intercellular adhesion molecule-l (ICAM-1) is an important molecule in immune and inflammatory responses. It is regulated by IL-1β, IL-4, TNF-α, and IFN-γ on many cell types. The aim of this study was to investigate the effects of IFN-γ and IL-4 on expression of ICAM-1 in cultured normal human nasal epithelial (NHNE) cells. Materials and Methods: NHNE cells were prepared by primary culture method of monolayer culture of dissociated cells from inferior turbinate mucosa. Primary cultured cells were characterized as an epithelial cell type by immunofluorescence assay and transmission electron microscopy. We analyzed the quantitative expression of ICAM-1 on cultured NHNE cells by treated with IFN-γ and IL-4 using fluorescence activated cell sorter (FACS). Results: Treatment of cultured NHNE cells with IFN-γ (1ng/ml) for 24 hours induced 4-fold increase of the surface ICAM-1 compared with constitutive expression by mean intensity fluorescence (MIF). However, IL-4 showed no effect even though the concentration of it was increased gradually. IFN-γ induced ICAM-1 expression according to the time course was significantly highest level at 24 hours. There were no significant changes in expression of ICAM-1 by IL-4 according to the time course. Conclusion: These findings suggest that IFN-γ is a potent ICAM-1 inducer, but IL-4 have no effects on expression of ICAM-1 on cultured NHNE cells. (J Clinical Otolaryngol 2000;11:261-266)