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집먼지 진드기 항원으로 자극한 사람 섬유아세포의 Cytokine mRNA 발현

유태현1,*, 이봉희1, 최창용1, 주호범1, 옥미선2, 김광혁3
Tai Hyun Yu1,*, Bong Hee Lee1, Chang Yong Choi1, Ho Bum Ju1, Mee Sun Ock2, Kwang Hyuk Kim3
Author Information & Copyright
1고신대학교 의과대학 이비인후과학교실
2기생충학교실
3미생물학교실
1Department of Otolaryngology, Kosin University, College of Medicine, Pusan, Korea
2Parasitology, Kosin University, College of Medicine, Pusan, Korea
3Microbiology, Kosin University, College of Medicine, Pusan, Korea
*교신저자: 유태현, 602-792 부산광역시 서구 암남동 34 고신대학교 의과대학 이비인후과학교실 전화: (051) 240-6247· 전송: (051) 245-8539 E-mail: cauent@netsgo.com.

© Copyright 1999 The Busan, Ulsan, Gyeoungnam Branch of Korean Society of Otolaryngology-Head and Neck Surgery. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Jun 15, 1999; Accepted: Jul 20, 1999

Published Online: May 31, 2020

ABSTRACT

Background and Objectives: It is well known that normal fibroblast can secrete a group of cytokines, namely IL-1 α IL-1 β IL-6, IL-7, IL-8 and GM-CSF. The fibroblast is a major component of connective tissue in the airway and has many immunologic roles. In case of allergic rhinitis, neutrophils, macrophages and lymphocytes are infiltrated in nasal mucosa and these cells secrete several kinds of cytokines. These reactions cause the activation of fibroblast growth abnormally and result in fibrosis. Secreted cytokines also cause inflammatory reaction of connective tissue in nasal mucosa. To identify the role of cytokines of fibrosis in allergic rhinitis, we tried to find the differences of cytokine secretion patterns and amounts between the normal and allergic rhinitis patient’s fibroblast which was originated from inferior turbinate of nasal cavity and primarily cultured in vitro. Materials and Methods: Cultured human nasal fibroblast was activated with saline, lipopolysaccharide (LPS,5 μg/ml) and house dust mite antigen (100 μg/ml) for 4 hours. Results: When normal fibroblast was treated with saline, mRNA expression of IL-1 β IL-6 and GM-CSF was confirmed. But the mRNA band of IL-6 was not found in the patient group. Although it was identified that mRNA expression of IL-1 β IL-8 and GM-CSF in both groups, IL-8 mRNA expression was distinct in patient fibroblast activated with LPS. When the normal fibroblast was activated with mite antigen, the expression of IL-1 β IL-6, IL-8 and GM-CSF expression was identified. But IL-8 expression was suppressed in the patient fibroblast. Conclusion: These results suggest that IL-1 β IL-6 and IL-8 could play as the triggering factors of inflammation and fibrosis in allergic rhinitis. (J Clinical Otolaryngol 1999;10:224–230)

Keywords: 집먼지 진드기; 알레르기성 비염; 섬유아세포; 사이토킨; mRNA
Keywords: House dust mite; Allergic rhinitis; Fibroblast; Cytokine; m RNA