집먼지 진드기 항원으로 자극한 사람 섬유아세포의 Cytokine mRNA 발현

Background and Objectives: It is well known that normal fibroblast can secrete a group of cytokines, namely IL-1 α IL-1 β IL-6, IL-7, IL-8 and GM-CSF. The fibroblast is a major component of connective tissue in the airway and has many immunologic roles. In case of allergic rhinitis, neutrophils, macrophages and lymphocytes are infiltrated in nasal mucosa and these cells secrete several kinds of cytokines. These reactions cause the activation of fibroblast growth abnormally and result in fibrosis. Secreted cytokines also cause inflammatory reaction of connective tissue in nasal mucosa. To identify the role of cytokines of fibrosis in allergic rhinitis, we tried to find the differences of cytokine secretion patterns and amounts between the normal and allergic rhinitis patient’s fibroblast which was originated from inferior turbinate of nasal cavity and primarily cultured in vitro. Materials and Methods: Cultured human nasal fibroblast was activated with saline, lipopolysaccharide (LPS,5 μg/ml) and house dust mite antigen (100 μg/ml) for 4 hours. Results: When normal fibroblast was treated with saline, mRNA expression of IL-1 β IL-6 and GM-CSF was confirmed. But the mRNA band of IL-6 was not found in the patient group. Although it was identified that mRNA expression of IL-1 β IL-8 and GM-CSF in both groups, IL-8 mRNA expression was distinct in patient fibroblast activated with LPS. When the normal fibroblast was activated with mite antigen, the expression of IL-1 β IL-6, IL-8 and GM-CSF expression was identified. But IL-8 expression was suppressed in the patient fibroblast. Conclusion: These results suggest that IL-1 β IL-6 and IL-8 could play as the triggering factors of inflammation and fibrosis in allergic rhinitis. (J Clinical Otolaryngol 1999;10:224–230)