Journal of Clinical Otolaryngology Head and Neck Surgery
The Busan, Ulsan, Gyeoungnam Branch of Korean Society of Otolaryngology-Head and Neck Surgery
원저

비용조직에서 산화질소합성효소들의 발현

박성국1,*, 김동균3, 허경욱1, 양영일2
Seong Kook Park1,*, Dong Kyoon Kim3, Kyung Wook Heo1, Young II Yang2
1인제대학교 의과대학 부산백병원 이비인후과학교실
2해부병리학교실
3김동균이비인후과의원
1Department of Otorhinolaryngology-Head & Neck Surgery, Inje University, Pusan Paik Hospital, Busan, Korea
2Pathology, College of Medicine, Inje University, Pusan Paik Hospital, Busan, Korea
3Dong Gyoon Kim ENT Clinic, Busan, Korea
*교신저자: 박성국, 614-735 부산광역시 부산진구 개금2동 633-165 인제대학교 의과대학 부산백병원 이비인후과학교실 전화: (051) 890-6379·전송: (051) 892-3831 E-mail: sinus4@chollian.net

© Copyright 2002 The Busan, Ulsan, Gyeoungnam Branch of Korean Society of Otolaryngology-Head and Neck Surgery. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Dec 10, 2001; Accepted: Mar 18, 2002

Published Online: May 31, 2020

ABSTRACT

Background and Objective: The pathogenesis of nasal polyp is poorly understood. Nitric oxide (NO) plays a major role in a number of physiologic function and may be cytotoxic in high concentrations. NO is formed by the oxidative deamination of L-arginine by nitric oxide synthases (NOS). Three NOS isoforms have been identified in human. Constitutive forms of NOS are present in vascular endothelial cells (Type III NOS, eNOS) and neurons in the brain and the peripheral nervous system (Type I NOS, nNOS) whereas the inducible form (Type II NOS, iNOS) is transcriptionally induced by cytokines in macrophages, neutrophils, mast cells, smooth-muscle cells, and fibroblasts. The aim of this study was to detect and localize three NOS isoforms expression in nasal polyp tissues, and compare these findings with inferior nasal turbinate tissues. Materials and Methods: The authors examined the expression and localization of three NOS isoforms in nasal mucosal specimens from patients undergone elective nasal turbinectomy (n=10) and nasal polypectomy (n=23). The mRNA expressions of three NOS isoforms were determined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) followed by Southern hybridization. The protein expression of three NOS isoforms were examined by immunohistochemistry. Statistics were analysed using Wilcoxon rank sum test. Results: Semi-quantitative RT-PCR Southern analysis of RNA obtained from 23 surgical specimens of nasal polyps demonstrated that the mRNA expressions of iNOS and eNOS were significantly increased in nasal polyps compared with inferior turbinates. The nNOS mRNA was similarly expressed in nasal polyps and inferior turbinates. The immunohistochemical studies revealed that the immunoreactivity to iNOS protein was mainly localized to epithelium, whereas eNOS protein to vascular endothelium, and nNOS protein to inflammatory cell, epithelium and vascular endothelium in all specimens reviewed. The high level of expression of three NOS isoforms in the nasal polyps were demonstrated in this study. Conclusion: The authors suggest that iNOS mRNA, eNOS mRNA and their product, nitric oxide may play an important role in the formation and growth of nasal polyps. (J Clinical Otolaryngol 2002;13:85-92)

Keywords: 산화질소합성효소; 비용; 역전사중합효소 연쇄반응/southern blot; 면역조직화학적염색
Keywords: Nitric oxide synthase; Nasal polyp; RT-PCR/southern blot; Immunohistochemistry