Journal of Clinical Otolaryngology Head and Neck Surgery
The Busan, Ulsan, Gyeoungnam Branch of Korean Society of Otolaryngology-Head and Neck Surgery
원저

진주종에서 증식표지인자의 발현

장경훈1, 도홍림1, 양훈식1, 홍영호1, , 김훈1, 김춘길1
Kyung Hoon Chang1, Hong Lim Do1, Chan Seung Hwang1, Hoon Shik Yang1, Young Ho Hong1, Hoon Kim1, Chun Gil Kim1
1중앙대학교 의과대학 이비인후과 학교실
1Department of Otolaryngology, College of Medicine, Chung Ang University, Seoul Korea

© Copyright 1996 The Busan, Ulsan, Gyeoungnam Branch of Korean Society of Otolaryngology-Head and Neck Surgery. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Published Online: May 31, 2020

ABSTRACT

Middle ear cholesteatoma is often invasive with consequent bone destruction. Inflammatory stimulation of the underlying connective tissue may be responsible for the dysrégulation and abnormal proliferative feature of the kératinocytes in cholesteatoma. Comparative investigations were performed to assess the epithelial cell kinetics of cholesteatoma and duditory meatal skin. Monoclonal antibody PCNA and Ki-67 immunohistochemical stainings were applied.

Specimens of cholesteatoma samples (n = 30) showed an average PCNA score (quotient of the PCNA positive cells and the total number of cells) of 26.6 ± 10.1% and an average Ki-67 score of 15.9 ± 7.2% . Auditory meatal skin (n = 8) revealed an average PCNA score of 8.2 ± 3. 4% and an average ki-67 score of 4.9 ±0.8%. The results of this study confirm a highly increase in the proliferation rate of cholesteatoma kératinocytes, which had an PCNA score that was 3.24 times higher than the score for kératinocyte of auditory meatal skin, Ki-67 score of cholesteatoma was 3.24 times higher than auditory meatal skin. Since the cholesteatoma possesses a varible degress of proliferative activities depending on its histologic composition, the formation and accumulation of the keratin due to the continuous epithelial growth probably are important element in inducing the inflammation. The results confirm that cholesteatoma is a hyperproliferative activity and indicate that PCNA and Ki-67 immunohistochemical stainings are valuable tool for assessing cell kinetics in cholesteatoma.

Keywords: Cholesteatoma; PCNA; Ki-67