Journal of Clinical Otolaryngology Head and Neck Surgery
The Busan, Ulsan, Gyeoungnam Branch of Korean Society of Otolaryngology-Head and Neck Surgery

중이 진주종 및 만성중이염에서 랑게르한스 세포에 관한 면역조직화학적 연구

이정헌1, 이재훈1, 소병수1, 강금위1, 조향정1, 문형배1
Jung Hun Lee1, Jae Hoon Lee1, Byung Soo Soh1, Keum Wee Kang1, Hyang Jeong Cho1, Hyung Bae Moon1
1원광대학교 의과대학 이비인후과학교실·병리학교실
1Department of Otolaryngology·Pathology, College of Medicine Wonkwang University

© Copyright 1995 The Busan, Ulsan, Gyeoungnam Branch of Korean Society of Otolaryngology-Head and Neck Surgery. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Published Online: May 31, 2020


Middle ear cholesteatoma is biologically invasive keratinizing squamous epithelial cyst that contains the desquamated debris. And it is clinically important entity because it causes destruction of adjacent bones and serious complications.

The pathogenesis and pathophysiology of cholesteatoma has been debated but there are basic theories of epithelial ingrowth and metaplasia of middle ear epithleium. Also there is concept that expanding process of desquamation causes bone destruction and that it may be due to proteolytic enzymes such as collagenase. Recent works suggest that Langerhans cells have an immunologic altering function and have a role of generating and maintaining the chronic inflammatory reacton which induces bone resorption.

The morphologic feature of cholesteatoma was similar to that of the tympanic membrane but there was a increase of Langerhans cells in cholesteatoma. Langerhans cells are the bone marrow-derived immune cells of the epidermis. They express la antigens and receptors for the Fc portion of IgG and complement components. Langerhans cells appear to recognize antigen and present them to T-lymphocyte and possibly macrophage.

The authors used immunohistochemical method to indentify and quantify distribution of Langerhans cells(S-100 protein), T-lymphocyte(HLA-DR, CD3) using monoclonal antibody techniques.

We compared distribution of Langerhans cells in cholesteatoma matrix, infected tympanic membrane and adjacent canal skin of chrnic otitis media of which were taken from patients during operation.

The obtained results were as follows :

  1. The Langerhans cells in cholesteatoma matrix increased significantly compared to those in chronic otitis media and post auricular skin. The Langerhans cells in chronic otitis media were more increased numbers than those in post auricular skin(p<0.05).

  2. The Langerhans cells and T lymphocytes with inflammatory reaction in subepithelial tissue of cholesteatoma were significantly higher than those chronic otitis media(p<0.05).

Keywords: Langerhans cells; Cholesteatoma; Chronic otitis media; Immunohistochemistry