Journal of Clinical Otolaryngology Head and Neck Surgery

The Busan, Ulsan, Gyeoungnam Branch of Korean Society of Otolaryngology-Head and Neck Surgery

J Clin Otolaryngol Head Neck Surg 1993; 4(1):64-67

pISSN: 1225-0244, eISSN: 2713-833X

DOI: https://doi.org/10.35420/jcohns.1993.4.1.64

원저

일차배양된 인체 청신경종양의 Schwann 세포의 냉동저장과 재배양

유영상¹, 이재담^*

Vital Freezing of Human Schwann Cells from Acoustic Tumor for Storage and Reculture

Young-Sang Yue¹, Jae-Dam Lee^*

¹울산대학교 의과대학 이비인후과학교실

^*생화학교실

¹Department of Otolaryngology, College of Medicine, University of Ulsan

^*Biochemistry, College of Medicine, University of Ulsan

© Copyright 1993 The Busan, Ulsan, Gyeoungnam Branch of Korean Society of Otolaryngology-Head and Neck Surgery. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Published Online: May 31, 2020

ABSTRACT

The procedure to recover viable and unaltered cells from frozen culture has been established for fibroblasts and lymphocytes etc. Vital freezing of Schwann cells from human acoustic tumors to reestablish cultures for future studies would also be of great importance.

We report a technique for vital freezing of human Schwann cells from acoustic tumor which enables to be recultured after long-term storage in refrigerator of — 138°C and evaluate cellular morphology, antigenic characteristic against S-100 protein and replicative propeties of Schwann cells on the bovine pituitary extract.

Keywords: Acoustic tumor; Schwann cell; Vital freezing; Reculture