진주종에서 증식표지인자의 발현

Middle ear cholesteatoma is often invasive with consequent bone destruction. Inflammatory stimulation of the underlying connective tissue may be responsible for the dysrégulation and abnormal proliferative feature of the kératinocytes in cholesteatoma. Comparative investigations were performed to assess the epithelial cell kinetics of cholesteatoma and duditory meatal skin. Monoclonal antibody PCNA and Ki-67 immunohistochemical stainings were applied.

Specimens of cholesteatoma samples (n = 30) showed an average PCNA score (quotient of the PCNA positive cells and the total number of cells) of 26.6 ± 10.1% and an average Ki-67 score of 15.9 ± 7.2% . Auditory meatal skin (n = 8) revealed an average PCNA score of 8.2 ± 3. 4% and an average ki-67 score of 4.9 ±0.8%. The results of this study confirm a highly increase in the proliferation rate of cholesteatoma kératinocytes, which had an PCNA score that was 3.24 times higher than the score for kératinocyte of auditory meatal skin, Ki-67 score of cholesteatoma was 3.24 times higher than auditory meatal skin. Since the cholesteatoma possesses a varible degress of proliferative activities depending on its histologic composition, the formation and accumulation of the keratin due to the continuous epithelial growth probably are important element in inducing the inflammation. The results confirm that cholesteatoma is a hyperproliferative activity and indicate that PCNA and Ki-67 immunohistochemical stainings are valuable tool for assessing cell kinetics in cholesteatoma.